FORIER TRANSFORM INFRARED SPECTROMETER (FTIR)

Fourier transformed infrared (FTIR) spectroscopy is a frequently used method in the basic sciences, health sciences and engineering fields by defining the bonds in the structure of molecules. The basis of this method is based on the absorption of the infrared rays, which are reduced on the intracellular bonds, by the vibrations and rotational movements of the bonds. However, this phenomenon is only observed in polar molecules with dipole moment.

 

IR spectrometer with ARUM can be used to irradiate solid state samples with potassium bromide (KBr) or directly by the ATR unit to obtain IR spectra. Direct IR spectra of liquid and gas samples can be obtained with ATR, attenuated total reflection unit, attenuated. With the diamond and germanium crystals used in the ATR unit, high-resolution IR spectra of different types of samples can be obtained, as well as temperature tests up to 300 ° C with the heating plate. IR spectrometry can be applied in almost every field as an indispensable device for both academic and industrial studies. In addition, thanks to a library of 22,000 spectra, some organic and inorganic molecules can be identified.

 

IR Device: Perkin Elmer Spectrum Two

ATR Unit: PIKE Gladi ATR Diamond and Crystals of Germanium

Spectrum Range: 8300 - 350 cm-1

 

Information to be Obtained by IR Analysis;

• Determination of intracellular bonds,
• Determination of molecular formula,
• Examination of intracellular functional groups,
• Unknown molecular assay with library scanning,
• Quantification of the components in the sample by quantitative program.

 

ULTRAVIOLET-VISIBLE LIGHT SPECTROPHOTOMETER (UV-VIS-NIR)

UV-VIS spectroscopy is a method commonly used in the identification of organic molecules, ions or complexes, including the wavelength range, including ultraviolet and visible light regions. The basis of this method is based on the increase in absorption (absorption) due to a decrease in the intensity of the beam after a beam is passed through a sample or reflected from a surface of a sample. Since the rays passing through or reflected from the sample are absorbed by each molecule with a specific wavelength, the molecular spectra can be determined by reference spectra.

 

The UV-VIS-NIR spectrophotometer within the ARUM includes the near-infrared (NIR) region along with the ultraviolet and visible light regions as the wavelength range and offers a much wider spectrum of analysis. Temperature-dependent tests (up to 60 ° C) can be realized with the integrated temperature accessories. In addition to this, solid state samples can be analyzed as well as liquid samples. UV-VIS-NIR spectrophotometry is the basis of choice in both academic and industrial fields and includes water, environment, medicine, chemistry, biology, biochemistry etc. indispensable in areas.

 

UV-VIS-NIR Device: Perkin Elmer Lambda 750 (Dual Beam)

Wavelength Range: 2500 - 190 nm

 

Information to be Obtained by UV-VIS-NIR Analysis;

• Determination of molecular formula
• Chemical reaction monitoring,
• Follow-up of reaction kinetics,
• Temperature dependent reaction monitoring,
• Turbidimetric studies.

 

 

FLUORESCENCE SPECTROPHOTOMETER

In molecular fluorescence spectroscopy, an atom or molecule stimulated by an electromagnetic beam emerges from the basic energy level to an upper energy level. The excited atom or molecule at the upper energy level is unstable and wants to return to the basic energy level by removing the excess energy it absorbs. At this stage, all or part of the excess energy can be separated as light emission from the atom or molecule. This phenomenon is generally referred to as luminescence, although it is referred to as fluorescence or phosphorescence, depending on the type of occurrence. In this way, luminescence characters of the studied samples can be determined.

 

Fluorescence and phosphorescence readings can be taken from liquid or solution samples with fluorescence spectrophotometer (or spectroflorimeter) within ARUM, but bone and bioluminescence studies can be performed.

 

FL Device: Perkin Elmer FL6500

Wavelength Range: 900 - 200 nm (excitation), 900 - 200 nm (emission)

 

Information Obtained by Spectrofluorimeter Analysis;

• Fluorescence, phosphorescence, luminescence measurements