NUCLEAR MAGNETIC RESONANCE (NMR) SPECTROMETRY

The spectroscopic method based on the principle that a nucleus which is held in an external magnetic field and whose spin quantum number is different from zero is resonated with a radio wave photon at the appropriate frequency value is called the Nuclear Magnetic Resonance (NMR) spectrometer. By using NMR information about the structure of the compound, binding properties, molecular formula and weight, molecular movements and structural arrangement of polymers can be obtained.

In the NMR laboratory of the ARUM Central Laboratory there is the NMR spectrometer of the JEOL ECZ500R (11.75 Tesla). Since both solid and liquid probes are present in our device, samples that are dissolved in any solvent as well as solid samples can be analyzed.

The superiority of solid NMR is that it allows analysis of samples which cannot be dissolved in any solvent. In the analysis of solid samples, it is possible to use the methods of magic angle spinning and CP (cross polarization). Unlike liquid NMR, solids can be analyzed by NMR, organic crystalline compounds, catalysts, zeolites, amorphous compounds, liquid crystals, polymers and biopolymers.

Information that can be obtained by NMR analysis;

The nature of the compound.
Structure and bonding.
The composition of the mixture components.
Atomic composition.
Molecular weight.
Molecular formula.
Polymer composition.
Polymer layout.
Molecular movement.
Molecular interchange process.
The process of intermolecular change.
 

NMR Instrument: JEOL ECZ500R

Magnet: High performance UltrashieldTM 500MHz superconducting magnet

Probe: 5 mm standard probe (¹H, ¹³C, ³¹P, ¹⁹F, ...)

Assays;

One-dimensional (¹H, ¹³C, ³¹P, ¹⁹F) and two-dimensional (COZY, HETCOR, NOESY, HMBC, HMQC,...) experiments

Sample Requirements;

Samples should be delivered with high purity and solvent indicated. For a minimum of 40 - 50 mg sample for ¹³C, a minimum of 10-20 mg sample is required for ¹H.