FLUORESCENCE SPECTROPHOTOMETER

In molecular fluorescence spectroscopy, an atom or molecule stimulated by an electromagnetic beam emerges from the basic energy level to an upper energy level. The excited atom or molecule at the upper energy level is unstable and wants to return to the basic energy level by removing the excess energy it absorbs. At this stage, all or part of the excess energy can be separated as light emission from the atom or molecule. This phenomenon is generally referred to as luminescence, although it is referred to as fluorescence or phosphorescence, depending on the type of occurrence. In this way, luminescence characters of the studied samples can be determined.

Fluorescence and phosphorescence readings can be taken from liquid or solution samples with fluorescence spectrophotometer (or spectroflorimeter) within ARUM, but bone and bioluminescence studies can be performed.

FL Device: Perkin Elmer FL6500

Wavelength Range: 900 - 200 nm (excitation), 900 - 200 nm (emission)

Information Obtained by Spectrofluorimeter Analysis;

• Fluorescence, phosphorescence, luminescence measurements.